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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Optimizing mesoderm progenitor selection and three-dimensional microniche culture allows highly efficient endothelial differentiation and ischemic tissue repair from human pluripotent stem cells

Fig. 5

High-throughput RNA sequencing data analysis of MESP1+ cells differentiated in 2D and 3D for 5 days. a Protocol for endothelial differentiation and RNA-seq. b Gene ontology analysis of top 500 upregulated genes and 500 downregulated genes in 3D GMs. c Q-PCR validation of representative endothelial marker genes enriched in 3D GM differentiation (n = 3, * p < 0.05, ** p < 0.005, *** p < 0.001, 3D versus 2D; t test)

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Stem Cell Research & Therapy

ISSN: 1757-6512