Fig. 2
From: Study of the reparative effects of menstrual-derived stem cells on premature ovarian failure in mice
MenSCs express high levels of mesodermal antigens and display multi-lineage capacities. a Flow cytometry histograms showing the expression level of the indicated cell surface markers on MenSCs. b Multipotent differentiation of MenSCs in vitro: osteogenic differentiation is indicated by Alizarin Red reactivity. Scale bar = 100 μm. c Multipotent differentiation of MenSCs in vitro: adipogenic differentiation was visualized using Oil Red staining, which reveals lipid droplets. Scale bar = 100 μm. d Differentiated MenSCs were identified using qPCR analysis to evaluate that expression of osteogenic (SPARC and Runx2) and adipogenic (FabP4 and PPARγ) markers. Expression levels of each gene were compared with the levels in undifferentiated MenSCs (data shown as mean ± SEM)