Fig. 4

Regulation of T cell subsets by treatment with ASC sup. The lymphocytes from LLNs were cultured with stimulated anti-CD3 antibody. Each 1 × 10⁶ lymphocytes per sample were analyzed after surface and internal staining with various antibodies. After gating with CD4⁺ T cells (a), IL-4⁺, IFN-γ⁺, IL-10⁺, CD25⁺, and Foxp3⁺, T cells were counted by FACS analysis (b). (OVA lymphocytes from LLN of OVA-Alum-induced airway inflammation mice, con sup + OVA lymphocytes from LLN of OVA-Alum-induced airway inflammation mice with con sup treatment, ASC sup + OVA lymphocytes from LLN of OVA-Alum-induced airway inflammation mice with ASC sup treatment. n = 5 mice per group, experiments were performed in triplicate)