Fig. 6

Recruitment of T_reg cells into the lung after ASC sup treatment. Paraffin sections of lungs from Foxp3-eGFP mice (green) were stained by immunofluorescence for CTLA-4 (red), and nuclei (DAPI, blue) representative pictures are shown, (white bar = 100 μm). (a) PBS lung of PBS-treated mice, (b) OVA lung of OVA-Alum-induced airway inflammation mice, (c) con sup + OVA lung of OVA-Alum-induced airway inflammation mice with con sup treatment, (d) ASC sup + OVA lung of OVA-Alum-induced airway inflammation mice with ASC sup treatment, Merge; merge of CTLA-4, GFP, and DAPI fields, Mag-Merge; magnification of Merge, Arrowhead; indicated CTLA4⁺Foxp3⁺ T cells. (e) Average ratio of CTLA4⁺Foxp3⁺ cells among total cells in the lung of each group was analyzed using the Image J program. (The value was calculated by three different authors who analyzed ten random spots of each slides). n = 5 mice per group, experiments were performed in triplicate