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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Pre-incubation with hucMSC-exosomes prevents cisplatin-induced nephrotoxicity by activating autophagy

Fig. 2

Human umbilical cord-derived mesenchymal stem cell exosomes (hucMSC-Ex) inhibit cisplatin-induced apoptosis and secretion of inflammatory cytokines in NRK-52E cells in vitro. a NRK-52E cells were stained with JC-1 dye and observed under fluorescent microscopy (JC-1 produces red fluorescence within the mitochondria as JC-1-aggregates while it emits green fluorescence when it leaks into the cytoplasm as JC-1-monomers; the shift between green and red is proportional to the mitochondrial membrane potential change) (200×, scale bar = 50 μm). b TUNEL assay was performed to detect the apoptotic cells (200×, scale bar = 50 μm). c Percentage of apoptotic cells. **P < 0.01, n = 3. d The expression of proliferating cell nuclear antigen (PCNA), BCL-XL, BCL-2, Bax, Cytochrome-c, cleaved Caspase-3, and GAPDH proteins was evaluated by Western blot. e Immunohistochemical analysis of tumor necrosis factor alpha (TNF-α) expression (200×, scale bar = 50 μm). f The expression of interleukin 1-β (IL1-β) and GAPDH proteins was evaluated by Western blot. NRK-52E cells treated as mentioned for the in vitro experiment were divided into four groups: 1) control; 2) phosphate-buffered saline (PBS); 3) hucMSC-Ex; and 4) human fetal lung fibroblast-1exosomes (HFL1-Ex)

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