Fig. 5

Immunosuppressive properties of CB-MSC. a-b Inhibitory effect of resting (a) and primed (b) LL-CBMSC on allogeneic CFSE-labeled PBMC. Cells were co-cultured at different PBMC:MSC ratios upon PBMC stimulation with anti-CD3 and rh-IL-2 for 6 days. Three different PBMC:MSC ratios were used ranging from 1:0.2 to 1:0.05. The 1:0 ratio represents the positive control (no MSC treatment and presence of PBMC antibody stimulation). Proliferation was assessed by CFSE dilution method on CD45+ cells. Each bar represents mean and SEM of two independent experiments with two different PBMC donors for each of four CB-MSC batches. c Proliferation ratio between the percentage of CD45+ proliferation at primed and resting conditions, at the different MSC doses. The differences were computed by two-way ANOVA, ^** p < 0.01, ^*** p < 0.001