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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Integrated analysis of hematopoietic differentiation outcomes and molecular characterization reveals unbiased differentiation capacity and minor transcriptional memory in HPC/HSC-iPSCs

Fig. 1

Genome-wide cellular characteristics of HPC/HSCs and OSKM-mediated reprogramming of HPC/HSCs. a Schematic showing the generation of HPC/HSC-iPSCs and the evaluation of hematopoietic differentiation potential and genome-wide memory status. b Hierarchical clustering of genome-wide gene expression patterns of HPC/HSCs, MEFs, R1 ESCs and iPSCs. The analysis of each cell line was repeated twice. c Distribution of DNA methylation (5mC) and histone modifications (H3K4me2, H3K4me3, and H3K27me3) in the TSS ± 2 kb regions of genes with high expression in HPC/HSCs. The normalized read counts in HPC/HSCs minus the counts in MEFs were used for the plots. d Distribution of DNA methylation (5mC) and histone modifications (H3K4me2, H3K4me3, and H3K27me3) in the TSS ± 2 kb regions of genes with low expression in HPC/HSCs. The normalized read counts in HPC/HSCs minus the counts in MEFs were used for the plots. e Western blot comparing the expression levels of the four reprogramming factors in OSKM-mediated reprogramming in the isolates of HPC/HSCs and MEFs. 4 N tetraploid complementation, HPC/HSC hematopoietic progenitor and stem cell, iPSC induced pluripotent stem cell, MEF mouse embryonic fibroblast, RNA-Seq RNA sequencing. f The read distribution of RNA-Seq, H3K27me3, H3K4me3, H3K4me2 ChIP-Seq and DNA methylation (5mC) in the region corresponding to the upstream region of the Dlk1-Dio3 locus in R1, MEFs, and HPC/HSCs. Scale bar, 30 kb

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