Fig. 1

Morphology and viability of rabbit amniotic-derived stem cells. a Cells cultured for 24 hours. Fibroblast-like cells adhered to the dish (arrows); 20× resolution. b Cells cultured for 72 hours. Intense contact between the cells (arrow) in high confluence (circle); 4× resolution. c Scanning electron microscopy. Cells during the fourth passage maintain a fibroblast-like morphology during the passages (arrows) with an elongated cytoplasm and central nucleus. d Flow cytometry analysis. Two distinct populations (circles) with different sizes and granularities. e, f Cellular metabolism testing during passages 4 and 8, respectively, by the MTT colourimetric method. Increased viability of the passage 4 cells. FSC Forward Scatter, SSC Side Scatter, D.O density optic