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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Administration of multipotent mesenchymal stromal cells restores liver regeneration and improves liver function in obese mice with hepatic steatosis after partial hepatectomy

Fig. 5

Donor MSCs show a pro-regenerative phenotype when it reaches the liver parenchyma. The in vitro expression of pro-fibrotic (α-SMA) and pro-regenerative (vimentin and desmin) markers was evaluated in donor MSCs before their administration. Representative micrographs of (a) individual immunoreactivity for α-SMA (Alexa Fluor 555 – red), vimentin (Alexa Fluor 488 – green) and desmin (Alexa Fluor 488 – green), and (b) co-expression of these markers. The in vivo expression of α-SMA, vimentin and desmin was evaluated by confocal microscopy in liver sections 2 days post MSCsGFP administration in normal and obese hepatectomized mice. Representative micrographs of (c) MSCsGFP/α-SMA (Alexa Fluor 488 – green/Alexa Fluor 555 – red), (d) MSCs-GFP/desmin (Alexa Fluor 488 –green/Alexa Fluor 555 – red) and (e) MSCs-GFP/vimentin (Alexa Fluor 488 – green/Alexa Fluor 555 – red) colocalization. In all cases nuclei were counterstained with DAPI (blue)

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