Fig. 5

Intestinal aquaporin 1 (AQP1) expression. a–d Immunohistochemistry of AQP1 (×100). There was high expression of AQP1 on the plasma membrane of the endothelial cells in the intestinal capillary in the control group (a). Compared with the control group, the expression of AQP1 decreased significantly in the acute necrotizing pancreatitis (ANP) group at 24 h (b). After treatment with mesenchymal stem cells (MSCs), the expression of AQP1 was higher than the expression in the ANP group at 24 h (c). Compared with the ANP group at 24 h, the expression of AQP1 was not significantly changed after treatment with culture media (d). e, f Western blotting of AQP1 protein in the small intestine. Values are presented as the mean ± SE (n = 10). *p < 0.01, versus the control group at 24 h and all ANP group time points; ^# p < 0.05, versus the ANP group at the same time point; ^☆ p < 0.05, versus the culture media-treated group at 24 h; as tested using a two-sample t test for comparing between two groups, and one-way ANOVA for comparing several groups