Fig 3

Expression of HGF, VEGF, and IGF-1 in the heart as determined by real-time quantitative PCR (a). Asterisks represent statistical difference compared with the DCM group (P < 0.05, ANOVA). b Histoimmunochemical stain of HGF (I and IV), VEGF (II and V), and IGF-1 (III and VI). Expression of these cytokines represented by yellow to brown stain was predominantly localized in the cytoplasm. Left panels (I-III) are heart tissues from the DCM control group, and right panels (IV-VI) from the high-dose group. Black bars = 50 μm. c Representative figure showing expression of VEGF in the heart detected by Western blot. β-actin was detected as a loading control. VEGF-m and VEGF-d represent VEGF monomer and dimer respectively. d Densitometric analysis of VEGF expression. Asterisks represent statistical difference compared with the DCM group (P < 0.05, ANOVA). DCM DCM control group, HD high-dose hUCMSC-treated group, HGF hepatocyte growth factor, IGF-1 insulin-like growth factor-1, LC low-dose hUCMSC-treated group, NC normal control group, SN supernatant-treated group, VEGF vascular endothelial growth factor