Fig. 1
Experimental design. a Rats were anesthetised and then submitted to bilateral renal arterial ischemia for 45 min. At 6 h after reperfusion, some rats were injected intraperitoneally with 1 × 106 huMSCs (IRI + huMSC group), whereas others went untreated (IRI group). Data were collected from four control, nine IRI and five IRI + huMSC rats. Plasma urea levels were determined daily over a 7-day period. On day 7, animals were euthanised and blood, urine and kidney samples collected from all. b In a second set of experiments, bilateral renal arterial ischemia was induced in 41 rats for 45 min, and after 6 h of reperfusion 20 rats were injected intraperitoneally with 1 × 106 huMSCs (IRI + huMSC group), four rats were injected with 1 × 106 aMSCs (IRI + aMSC group) and 17 rats went untreated (IRI group). On day 2, 22 rats were euthanised (IRI, n = 8; IRI + huMSC, n = 10; IRI + aMSC, n = 4). Twelve rats were euthanised on day 7 (IRI, n = 6; IRI + huMSC, n = 6), and seven rats were euthanised on day 49 (IRI, n = 3; IRI + huMSC, n = 4). Arrows indicate euthanasia, when blood, urine and kidney samples were collected from all animals. c Table indicating the experiments run at each time point in each sample collected from rats in the control, IRI and IRI + huMSC groups. d Table indicating the experiments run on day 2 for the IRI + aMSC group. aMSC adipose-derived mesenchymal stromal cell, D2 post-IRI day 2, D49 post-IRI day 49, D7 post-IRI day 7, huMSC human umbilical cord-derived mesenchymal stromal cell, IRI ischaemia/reperfusion injury, PCNA proliferating cell nuclear antigen, qPCR quantitative polymerase chain reaction