Table 2 Sequences of primers against chondrogenic markers in RT-PCR
Target name | Direction | Primer sequence | Size |
|---|---|---|---|
SOX9 | Forward | GAACGCACATCAAGACGGAG | 631 |
Reverse | TCTCGTTGATTTCGCTGCTC | ||
ACAN | Forward | TGAGGAGGGCTGGAACAAGTACC | 349 |
Reverse | GAGGTGGTAATTGCAGGGAACA | ||
COL2A1 | Forward | TTCAGCTATGGAGATGACAATC | 472 |
Reverse | AGAGTCCTAGAGTGACTGAG | ||
COMP | Forward | CAACTGTCCCCAGAAGAGCAA | 588 |
Reverse | TGGTAGCCAAAGATGAAGCCC | ||
COL1A1 | Forward | CCCCTGGAAAGAATGGAGATG | 148 |
Reverse | TCCAAACCACTGAAACCTCTG | ||
COL10 | Forward | CAGTCATGCCTGAGGGTTTT | 196 |
Reverse | GGGTCATAATGCTGTTGCCT | ||
GAPDH | Forward | GAATGGGCAGCCGTTAGGAA | 414 |
Reverse | GACTCCACGACGTACTCAGC |