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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Laminin differentially regulates the stemness of type I and type II pericytes

Fig. 5

Laminin inhibits adipogenic differentiation of type I pericytes. a Diagram of experimental design for adipogenic differentiation of type I pericytes. b Immunocytochemical analysis of perilipin (green) and Oil red O (red) staining in wild-type (WT) and laminin γ1flox/flox:Pdgfrβ-Cre+(PKO) type I pericytes in the presence of saline (Sal) or laminin-111 (Ln). c Quantification of perilipin (green) expression in type I pericytes after adipogenic differentiation. n = 4. d Percentage of Oil Red O+ (red) area in type I pericytes after adipogenic differentiation. n = 4. e Quantitative RT-PCR analyses of C/EBPα, C/EBPβ, C/EBPδ, KROX20, KLF5, and FABP4 expression in type I pericytes 4 days after adipogenic differentiation. n = 4. f Diagram of experimental design for long-term adipogenic differentiation of type II pericytes. g Immunocytochemical analysis of perilipin (magenta) and Oil red O (red)/hematoxylin (brown) staining in WT and PKO type II pericytes in the presence of sal or Ln. h Quantification of perilipin (magenta) expression in type II pericytes after adipogenic differentiation. n = 4. i Percentage of Oil Red O+ (red) area in type II pericytes. n = 4. j Quantitative RT-PCR analyses of C/EBPα, C/EBPβ, C/EBPδ, KROX20, KLF5, and FABP4 expression in type II pericytes 4 days after adipogenic differentiation. n = 4. Data are shown as mean ± SD. *p < 0.05, **p < 0.01 versus WT or WT + Sal; ## p < 0.01 versus PKO + Sal. Scale bars = 100 μm

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