Fig. 7
From: Laminin differentially regulates the stemness of type I and type II pericytes
Laminin promotes myogenic differentiation of type II pericytes. a Diagram of experimental design for myogenic differentiation. b Immunocytochemical analysis of S-Myosin (red) in wild-type (WT) and laminin γ1flox/flox:Pdgfrβ-Cre+(PKO) type I pericytes in the presence of saline (Sal) or laminin-111 (Ln). c Quantification of S-Myosin (red) expression in type I pericytes after myogenic differentiation. n = 4. d Quantitative RT-PCR analyses of Pax7, MyoD, Myf5, Mrf4, myogenin (Myog), and S-Myosin expression in type I pericytes 4 days after myogenic differentiation. n = 4. e Immunocytochemical analysis of S-Myosin (red) in WT and PKO type II pericytes in the presence of sal or Ln. f Quantification of S-Myosin (red) expression in type II pericytes after myogenic differentiation. n = 4. **p < 0.01 versus WT + Sal. g Quantitative RT-PCR analyses of Pax7, MyoD, Myf5, Mrf4, Myog, and S-Myosin expression in type II pericytes 4 days after myogenic differentiation. n = 4. h Western blots and quantification of Pax7, Myf5, and Myog expression in WT and PKO type II pericytes 7 days after myogenic differentiation. GAPDH was used as a loading control. n = 4–5. Data are shown as mean ± SD. # p < 0.05 versus PKO + Sal; *p < 0.05, **p < 0.01, ***p < 0.001 versus WT. Scale bars = 100 μm in b and 50 μm in d