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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: An effective ex-vivo approach for inducing endothelial progenitor cells from umbilical cord blood CD34+ cells

Fig. 5

Transplantation of human EPCs/ECs into the livers of NOD/SCID mice. a Acute liver injury was induced in mice by an intraperitoneal injection of 100 mg/kg MCT in the control, trans-7d, trans-14d, and trans-28d groups. EPCs/ECs infected with GFP lentivirus were transplanted into mice in the blank, trans-7d, trans-14d, and trans-28d groups (6 × 106 cells in 100 μl PBS per mouse) via the hepatic portal vein while the control group mice were treated with 100 μl PBS. At days 7, 14, and 28 after transplantation, mice in the trans-7d, trans-14d, and trans-28d groups were sacrificed by cervical dislocation, respectively, hepatic tissues were obtained to make cryosections (20 μm), and the GFP-positive cells were detected using immunofluorescent staining. In the control and blank groups, mice were sacrificed and hepatic tissues were analyzed 7 days after transplantation. Scale bar = 50 μm. b Percentages of GFP-positive cells in the mouse livers were analyzed by ImageJ software. Twenty-five cryosections per mouse (n = 8 each group) were calculated. Data are mean ± SD. **P < 0.01. DAPI 4,6-diamidino-2-phenylindole, GFP green fluorescent protein

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