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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Two-photon FLIM of NAD(P)H and FAD in mesenchymal stem cells undergoing either osteogenic or chondrogenic differentiation

Fig. 3

Redox ratio of FAD/NAD(P)H in differentiating MSCs. a Fluorescence and optical redox images of undifferentiated MSCs and MSCs during osteogenic and chondrogenic differentiation. Fluorescence of FAD is shown in green, fluorescence of NAD(P)H in blue. b Dynamics of the fluorescence intensity of NAD(P)H and FAD in undifferentiated MSCs and MSCs during osteogenic and chondrogenic differentiation, mean ± SD. *Statistically significant difference on NAD(P)H with undifferentiated MSCs on the same day; #statistically significant difference on FAD with undifferentiated MSCs on the same day c Dynamics of the ratio in undifferentiated MSCs and MSCs during osteogenic and chondrogenic differentiation, mean ± SD. *Statistically significant difference with undifferentiated MSCs on the same day. For NAD(P)H: excitation - 750 nm, detection - 455–500 nm; for FAD: excitation - 900 nm, detection - 500–550 nm. The image size is 213 × 213 μm (1024 × 1024 pixels). FAD oxidized form of flavin adenine dinucleotide, MSCs mesenchymal stem cells, NAD(P)H reduced nicotinamide adenine dinucleotide (phosphate)

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Stem Cell Research & Therapy

ISSN: 1757-6512