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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Two-photon FLIM of NAD(P)H and FAD in mesenchymal stem cells undergoing either osteogenic or chondrogenic differentiation

Fig. 5

FLIM of NAD(P)H in MSCs during chondrogenic differentiation. a Pseudocolor-coded images of the free (t1) and protein-bound (t2) forms of NAD(P)H. Field of view 213*213 μm (512*512 pixels). b Dynamics of the fluorescence lifetimes of free and protein-bound forms of NAD(P)H and fluorescence lifetime contributions of the protein-bound forms of NAD(P)H in MSCs. Mean ± SD. *Statistically significant difference with undifferentiated MSCs on the same day. P values are shown. MSCs mesenchymal stem cells, NAD(P)H reduced nicotinamide adenine dinucleotide (phosphate)

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