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Table 1 The real-time polymerase chain reaction (RT-PCR) results showed differential mRNA and lncRNA expression levels during osteogenic differentiation of bone marrow mesenchymal stem cells

From: Differential long noncoding RNA/mRNA expression profiling and functional network analysis during osteogenic differentiation of human bone marrow mesenchymal stem cells

 

RT-PCR results

Microarray results

 

Gene symbol

0 days

7 days

7-day/0-day (fold-change)

Regulation

XR_111050

1.00 ± 0.063

32.76 ± 4.29**

14.13

Up

NR_045555

1.00 ± 0.11

4.08 ± 0.28**

5.14

Up

NR_002196

1.00 ± 0.089

10.08 ± 1.53**

4.7

Up

NR_024593

1.00 ± 0.24

2.16 ± 0.29**

4.23

Up

NR_003255

1.00 ± 0.11

3.78 ± 0.27**

2.68

Up

NR_027621

1.00 ± 0.081

2.86 ± 0.18**

1.88

Up

NR_034115

1.00 ± 0.19

0.20 ± 0.051**

0.27

Down

NR_037595

1.00 ± 0.27

0.57 ± 0.062**

0.32

Down

NR_034181

1.00 ± 0.085

0.39 ± 0.072**

0.49

Down

FGF7

1.00 ± 0.10

14.42 ± 1.27**

17.86

Up

FOXO1

1.00 ± 0.14

4.33 ± 0.46**

3.97

Up

STAT2

1.00 ± 0.19

2.61 ± 0.42**

2.8

Up

TMEM156

1.00 ± 0.12

0.25 ± 0.059**

0.084

Down

FOXM1

1.00 ± 0.21

0.17 ± 0.031**

0.27

Down

FOXC2

1.00 ± 0.19

0.59 ± 0.082**

0.41

Down

  1. GAPDH was used as an internal control. The results represent mean ± standard deviation from three independent experiments (mean value of gene expression/GAPDH at day 0 was set as 1). Student’s t test was performed to determine statistical significance; **p < 0.01