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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Mesenchymal stem cells alleviate Japanese encephalitis virus-induced neuroinflammation and mortality

Fig. 5

MSC transplantation alleviates virus-induced disintegration of the BBB and improves the expression of ZO-1 in the mouse brain. Mice from each group were euthanized to detect the integrity of the BBB at 6 dpi. a BBB permeability was evaluated by the uptake of Evans blue dye assay (EB), and the brains were removed and photographed. These pictures show extravasated EB staining of the whole brain at 6 dpi. b The absorbance of EB in the brain was measured at 620 nm using a spectrophotometer and recorded as nanograms of dye per gram of brain tissue (PBS n= 2, JEV n= 3, JEV + MSC n= 3). c The expression of the tight junction protein zonula occludens-1 (ZO-1) in the brain sections was assessed by immunofluorescence. d The fluorescence intensity of ZO-1 staining was analyzed using ImageJ and showed that MSC treatment increased the level of ZO-1 compared with the JEV-infected group (PBS n= 3, JEV n= 6, JEV + MSC n= 6; three sections per mouse, five fields per section). **P < 0.01, ***P < 0.001. JEV Japanese encephalitis virus, MSC mesenchymal stem cell, PBS phosphate-buffered saline

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