|
Markers
|
Forward primer
|
Reverse primer
|
Tm
|
Product length
|
|---|
|
β-Actin
|
CATGTACGTTGCTATCCAGGC
|
CTCCTTAATGTCACGCACGAT
|
60
|
250bp
|
|
VEGF-A
|
GCAGAATCATCACGAAGTGG
|
GCATGGTGATGTTGGACTCC
|
60
|
234bp
|
|
TGF-β1
|
CAGATCCTGTCCAAGCTG
|
TCGGAGCTCTGATGGTGTT
|
56
|
270bp
|
|
Ang-1
|
GCTTACCAGATTCACACTGTTCC
|
TTGCTACCTTGCCAACAACAACTG
|
60
|
612bp
|
|
IL-6
|
CACAGACAGCCACTCACCTC
|
TTTTCTGCCAGTGCCTCTTT
|
60
|
137bp
|
|
HGF
|
ATGCATCCAAGGTCAAGGAG
|
TTCCATGTTCTTGTCCCACA
|
61
|
349bp
|
|
SDF-1α/CXCL12
|
TGCCAGAGCCAACGTCAAG
|
CAGCCGGGCTACAATCTGAA
|
60
|
73bp
|
|
VEGFR1
|
CGTAGAGATGTACAGTGAAA
|
GGTGTGCTTATTTGGACATC
|
55
|
306bp
|
|
VEGFR2
|
TTACAGATCTCCATTTATTGC
|
TTCATCTCACTCCCAGACT
|
60
|
498bp
|
|
VEGFR3
|
CTGGACCGAGTTTGTGGAGG
|
GTCACATAGAAGTAGATGAGCCG
|
60
|
138bp
|
-
Tm melting temperature, VEGF-A vascular endothelial growth factor A, TGF-β transforming growth factor beta, Ang-1 angiopoietin-1, IL interleukin, HGF hepatocyte growth factor, SDF-1 stromal-derived factor-1, VEGFR1 vascular endothelial growth factor receptor 1, VEGFR2 vascular endothelial growth factor receptor 2, VEGFR3 vascular endothelial growth factor receptor 3
