Development of a surrogate potency assay to determine the angiogenic activity of Stempeucel®, a pooled, ex-vivo expanded, allogeneic human bone marrow mesenchymal stromal cell product

Table 1 Primer list- Real Time PCR

Markers	Forward primer	Reverse primer	Tm	Product length
β-Actin	CATGTACGTTGCTATCCAGGC	CTCCTTAATGTCACGCACGAT	60	250bp
VEGF-A	GCAGAATCATCACGAAGTGG	GCATGGTGATGTTGGACTCC	60	234bp
TGF-β1	CAGATCCTGTCCAAGCTG	TCGGAGCTCTGATGGTGTT	56	270bp
Ang-1	GCTTACCAGATTCACACTGTTCC	TTGCTACCTTGCCAACAACAACTG	60	612bp
IL-6	CACAGACAGCCACTCACCTC	TTTTCTGCCAGTGCCTCTTT	60	137bp
HGF	ATGCATCCAAGGTCAAGGAG	TTCCATGTTCTTGTCCCACA	61	349bp
SDF-1α/CXCL12	TGCCAGAGCCAACGTCAAG	CAGCCGGGCTACAATCTGAA	60	73bp
VEGFR1	CGTAGAGATGTACAGTGAAA	GGTGTGCTTATTTGGACATC	55	306bp
VEGFR2	TTACAGATCTCCATTTATTGC	TTCATCTCACTCCCAGACT	60	498bp
VEGFR3	CTGGACCGAGTTTGTGGAGG	GTCACATAGAAGTAGATGAGCCG	60	138bp

Tm melting temperature, VEGF-A vascular endothelial growth factor A, TGF-β transforming growth factor beta, Ang-1 angiopoietin-1, IL interleukin, HGF hepatocyte growth factor, SDF-1 stromal-derived factor-1, VEGFR1 vascular endothelial growth factor receptor 1, VEGFR2 vascular endothelial growth factor receptor 2, VEGFR3 vascular endothelial growth factor receptor 3