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Table 1 Primer list- Real Time PCR

From: Development of a surrogate potency assay to determine the angiogenic activity of Stempeucel®, a pooled, ex-vivo expanded, allogeneic human bone marrow mesenchymal stromal cell product

Markers

Forward primer

Reverse primer

Tm

Product length

β-Actin

CATGTACGTTGCTATCCAGGC

CTCCTTAATGTCACGCACGAT

60

250bp

VEGF-A

GCAGAATCATCACGAAGTGG

GCATGGTGATGTTGGACTCC

60

234bp

TGF-β1

CAGATCCTGTCCAAGCTG

TCGGAGCTCTGATGGTGTT

56

270bp

Ang-1

GCTTACCAGATTCACACTGTTCC

TTGCTACCTTGCCAACAACAACTG

60

612bp

IL-6

CACAGACAGCCACTCACCTC

TTTTCTGCCAGTGCCTCTTT

60

137bp

HGF

ATGCATCCAAGGTCAAGGAG

TTCCATGTTCTTGTCCCACA

61

349bp

SDF-1α/CXCL12

TGCCAGAGCCAACGTCAAG

CAGCCGGGCTACAATCTGAA

60

73bp

VEGFR1

CGTAGAGATGTACAGTGAAA

GGTGTGCTTATTTGGACATC

55

306bp

VEGFR2

TTACAGATCTCCATTTATTGC

TTCATCTCACTCCCAGACT

60

498bp

VEGFR3

CTGGACCGAGTTTGTGGAGG

GTCACATAGAAGTAGATGAGCCG

60

138bp

  1. Tm melting temperature, VEGF-A vascular endothelial growth factor A, TGF-β transforming growth factor beta, Ang-1 angiopoietin-1, IL interleukin, HGF hepatocyte growth factor, SDF-1 stromal-derived factor-1, VEGFR1 vascular endothelial growth factor receptor 1, VEGFR2 vascular endothelial growth factor receptor 2, VEGFR3 vascular endothelial growth factor receptor 3