In vitro generation of Sertoli-like and haploid spermatid-like cells from human umbilical cord perivascular cells

Table 1 FTM HUCPVCs and term HUCPVCs express testis niche-associated growth factors

Gene	Full gene name	Average Ct		Standard deviation		Fold-change (FTM/term)	p value
Gene	Full gene name	Term HUCPVC	FTM HUCPVC	Term HUCPVC	FTM HUCPVC	Fold-change (FTM/term)	p value
GDNF	Glial cell-derived neurotrophic factor	27	27	0.6	0.4	1.08	0.975
FGF2	Fibroblast growth factor 2	22	23	0.2	1.1	0.66	0.693
FGF1	Fibroblast growth factor 1	25.3	26.3	0.32	0.73	0.5	0.037
LIF	Leukemia inhibitory factor	24	24	1.3	0.7	0.95	0.713
BMP6	Bone morphogenetic protein 6	27	27	0.8	1.8	1.39	0.516
BMP4	Bone morphogenetic protein 4	26	28	0.5	2.8	0.28	0.296
TGFB1	Transforming growth factor beta 1	23	23	0.2	0.3	0.83	0.399
VEGFA	Vascular endothelial growth factor A	24	24	0.5	0.9	1.16	0.693

Selected data from the growth factor qPCR array (Sabiosciences) showing transcripts for factors involved in testicular development and function, which are moderately expressed in human umbilical cord perivascular cells (HUPVCs)
Ct values were normalized to the housekeeping genes on the array (GAPDH, RPL13A, HPRT1)
n = 3 independent lines of first trimester (FTM) HUCPVCs and term HUCPVCs at passage 4 following expansion in α-minimum essential medium + 10% fetal bovine serum

ISSN: 1757-6512