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Table 1 FTM HUCPVCs and term HUCPVCs express testis niche-associated growth factors

From: In vitro generation of Sertoli-like and haploid spermatid-like cells from human umbilical cord perivascular cells

Gene Full gene name Average Ct Standard deviation Fold-change (FTM/term) p value
Term HUCPVC FTM HUCPVC Term HUCPVC FTM HUCPVC
GDNF Glial cell-derived neurotrophic factor 27 27 0.6 0.4 1.08 0.975
FGF2 Fibroblast growth factor 2 22 23 0.2 1.1 0.66 0.693
FGF1 Fibroblast growth factor 1 25.3 26.3 0.32 0.73 0.5 0.037
LIF Leukemia inhibitory factor 24 24 1.3 0.7 0.95 0.713
BMP6 Bone morphogenetic protein 6 27 27 0.8 1.8 1.39 0.516
BMP4 Bone morphogenetic protein 4 26 28 0.5 2.8 0.28 0.296
TGFB1 Transforming growth factor beta 1 23 23 0.2 0.3 0.83 0.399
VEGFA Vascular endothelial growth factor A 24 24 0.5 0.9 1.16 0.693
  1. Selected data from the growth factor qPCR array (Sabiosciences) showing transcripts for factors involved in testicular development and function, which are moderately expressed in human umbilical cord perivascular cells (HUPVCs)
  2. Ct values were normalized to the housekeeping genes on the array (GAPDH, RPL13A, HPRT1)
  3. n = 3 independent lines of first trimester (FTM) HUCPVCs and term HUCPVCs at passage 4 following expansion in α-minimum essential medium + 10% fetal bovine serum