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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton’s jelly mesenchymal stem cells (WJ-MSC)

Fig. 3

Netrin-1 promotes angiogenesis in HUVEC. a Representative images of HUVEC tubule assay. Cells were exposed for 4 h to endothelial basal media (EBM), endothelial growth media (EGM), IgG (internal antibody control, 2 μg/mL), 2F5 [a drug targeting Netrin-1 (NTN-1), 2 μg/mL] and anti-NTN-1 antibody (R&D Systems, 2 μg/mL), in absence or presence of recombinant human NTN-1 (10 ng/mL), scale bar = 15 μm. b Quantified data correspond to the mean ± S.E.M. (n = 4, * p < 0.05 vs. EBM, γ p < 0.05 vs. NTN-1, α p < 0.05 vs. IgG + NTN-1)

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