Fig. 4

Netrin-1, secreted by WJ-MSC, promotes angiogenesis in HUVEC. a Representative images of HUVEC tubule assay treated as indicated. Cells were exposed for 4 h to DMEM, endothelial growth media (EGM), WJ-MSC-conditioned media (CM), 2F5 [a drug targeting Netrin-1 (NTN-1), 0.5 μg/μL] and CBO (a VEGF-receptor inhibitor, 20 μM), scale bar = 15 μm. b Quantified data correspond to the mean ± S.E.M. (n = 3, ^* p < 0.05 vs. DMEM, ^β p < 0.05 vs. EGM, ^Φ p < 0.05 vs. CM)