Fig. 5

CTLA-4 expression in CD4⁺CD117⁺CD44⁺ thymocytes by RT-PCR and real-time RT-PCR. CD4⁺CD117⁺CD44⁺ and CD4⁺ SP cells sorted from the thymocytes of C57BL/6 mice (a). Total RNA extracted for RT-PCR and real-time RT-PCR analysis of CTLA-4 expression. b CTLA-4 expression in thymocytes by RT-PCR. c Integrated density value (IDV) for CTLA-4 transcripts quantified and normalized to β-actin. Representative results from one of three independent experiments. d Data pooled from three independent experiments. e Total RNA isolated for measuring CTLA-4 mRNA by real-time RT-PCR. Data are fold-induction relative to β-actin and representative results from one of three independent experiments. ***p < 0.001