Fig. 7

Nanog expression in CD117⁺CD44⁺ cells measured with RT-PCR and real-time RT-PCR. a Nanog in the thymocytes by RT-PCR. b Integrated density value (IDV) for the Nanog transcripts quantified and normalized to those of β-actin. c Total RNA isolated for measuring Nanog mRNA using real-time RT-PCR. Data presented as fold-induction relative to β-actin. Data represent one of three independent experiments. ***p < 0.001