Fig. 7

Effects of hAEC-conditioned medium treatment on primary microglia isolated from P14 animals, relative to controls treated with ultra-culture medium alone. a Percentage of microglia expressing the M1 marker CD86 and M2 marker CD206; 24-h exposure to hAEC-conditioned medium significantly reduced the percentage of CD86 expressing microglia. b Soluble factors from hAECs do not affect microglia proliferation after LPS stimulation at 24, 48 or 72 h. c Representative FACS plots illustrating the marked increase in pHrodo⁺ cells after 24-h hAEC co-culture. d After a 24-h co-culture of primary microglia with hAECs, the number of pHrodo⁺ cells increased significantly. *p < 0.05, **p < 0.01, Student’s t test. hAEC human amnion epithelial cell, CM conditioned medium