Fig. 4

Induced pluripotent stem cell-mesenchymal stem cells (iPSC-MSCs) induced the generation of IL-10-producting DCs under LPS stimulation. Allogenic peripheral blood mononuclear cells (PBMCs) were stained with carboxyfluorescein diacetate succinimidyl diester (CFSE) and co-cultured for 72 h with mature dendritic cells (mDCs) or iPSC-MSC-DCs, and lymphocyte proliferation was assessed by flow cytometry. a Representative experiments assessing lymphocyte proliferation. b The effects of iPSC-MSCs on the function of mDCs in lymphocyte proliferation. c Phagocytic ability of the DCs was analyzed by flow cytometry. d Statistical analysis of the phagocytic ability of DCs (the mean fluorescence intensity (MFI) of dextran). e Interleukin-10 (IL-10) and f IL-12p70 levels produced by mDCs, iPSC-MSC-DCs, or 7d-iPSC-MSC-DCs. The findings were confirmed using U-iPSC-MSCs and A-iPSC-MSCs and the data represent the means ± SD of six independent experiments using U-iPSC-MSCs. *P < 0.05, **P < 0.01, ***P < 0.001. FITC fluorescein isothiocyanate, iDCs immature DCs, iPSC-MSC-DCs DCs co-cultured with iPSC-MSCs from day 5 to day 7 in the presence of GM-CSF, IL-4, and LPS, 7d-iPSC-MSC-DCs DCs co-cultured with iPSC-MSCs from day 0 to day 7 under conditions of general DC culture in Figs. 2a and 3a