Table 1 Primers sequences for gene expression analysis using real-time PCR
Genes | Accession number | Primer sequence (5′-3′) | Product size (bp) |
|---|---|---|---|
Collagen-I | NM_000088.3 | Forward: AAGGTGTTGTGCGATGACG Reverse: TGGTCGGTGGGTGACTCTG | 116 |
Collagen-IV | NM_001303110.1 | Forward: AGGAGACTTCGCCACCAA Reverse: GGTCCTGTGCCTATAACAATT | 264 |
Vimentin | NM_003380.3 | Forward: CTGAACCTGAGGGAAACTAA Reverse: AGAAAGGCACTTGAAAGCT | 231 |
Connexin-43 | NM_000165.4 | Forward: CAGTCTGCCTTTCGTTGT Reverse: CTCTTCCTTTCGCATCAC | 168 |
Vitronectin | NM_000638.3 | Forward: CAAAGGCTACCGTTCACAA Reverse: AGACACTCTGGATGGGTTCA | 182 |
Elastin | NM_001278918.1 | Forward: CGCTGGTGCTCTTATCTTC Reverse: GCTTCAGGTGCTTGGGTA | 176 |
β-Actin | NM_005559.3 | Forward: AGCGAGCATCCCCCAAAGTT Reverse: GGGCACGAAGGCTCATCATT | 285 |