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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Repeated intra-articular injection of allogeneic mesenchymal stem cells causes an adverse response compared to autologous cells in the equine model

Fig. 1

Synovial fluid cytology box plots of total nucleated cell count (TNCC), percentage of neutrophils (PMN), total protein concentration (TP), percentage of lymphocytes and percentage of monocytes. Repeated measures analysis of variance (ANOVA) was used to compare temporal changes in the cytologic outcome variables for each pair of treatment groups (AUTO to FBS and AUTO to ALLO), with horse considered a random effect. These analyses were performed using PROC MIXED, and an autoregressive correlation structure was specified. Treatment group (AUTO, ALLO or FBS), time point, and their interaction were included as factors in the ANOVA. Following the first intra-articular MSC injection, the change in total nucleated cell count (TNCC) over time was not significantly different between either the AUTO and FBS groups or the AUTO and ALLO groups. Following the second joint injection 4 weeks later, there was a significant difference in the TNCC over time between the AUTO and FBS groups (p = 0.03; FBS was higher) and the AUTO and ALLO groups (p = 0.009; ALLO was higher). Specifically, there was a treatment effect at day 30 for the AUTO and FBS groups (p = 0.0007; FBS was higher) and the AUTO and ALLO groups (p = 0.0009; ALLO was higher). Other cytology parameters did not vary over time for either group after either injection. FBS autologous cells not depleted of fetal bovine serum (FBS), AUTO autologous cells depleted of FBS, ALLO allogeneic cells

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