Fig. 3
From: Exploiting decellularized cochleae as scaffolds for inner ear tissue engineering
Comparison of lipid content via scanning electron microscopy. No lipid content was detected in cochlear sections that were decellularized (DCs). No cells were present on the basilar membrane of DCs. Native cochlea (NC) sections displayed positive backscatter signal for lipid content. The Everhart-Thornley detector (ETD) is a standard secondary electron detector used in scanning electron microscopy, while the circular backscatter (CBS) is a backscatter detector. When tissues are stained with osmium tetroxide (OT), the OT embeds within lipids, and gives off a strong backscatter signal. Native cochlea scale bar = 50 μm. Decelled cochlea scale bar = 20 μm