Fig. 1From: Influence of temperature fluctuations during cryopreservation on vital parameters, differentiation potential, and transgene expression of placental multipotent stromal cellsCharacterization of primary cell culture derived from placenta for MSC origin. a RT-PCR analysis for expression of typical MSC markers: cells provide a signal for CD90, CD73, CD105, CD106, and CD166 genes while being negative for CD34, CD45, and MHC class II. b Differentiation potential of primary cell culture derived from placenta into lineages of MSC origin: adipogenic (Oil Red O staining); chondrogenic (Alcian blue staining); and osteogenic (Von Kossa staining). Cells expanded in standard MSC culture medium were considered as a negative control. Scale bars = 50 μmBack to article page