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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Influence of temperature fluctuations during cryopreservation on vital parameters, differentiation potential, and transgene expression of placental multipotent stromal cells

Fig. 3

Cryomicroscopic analysis of crystal formation during temperature fluctuations. Freezing of placental MSCs from 0 °C to –100 °C and thawing from –100 °C to –6.7 °C from left to right, top to bottom (the temperature is indicated on each image). a Samples at –80 °C during the first cycle of temperature fluctuations; b samples at –100 °C during the last cycle of temperature fluctuations; c samples at –80 °C during the last cycle of temperature fluctuations. Arrows indicate the change of inclusions. Scale bars = 20 μm

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