Fig. 5

Adhesive properties of the cells 24 h after cryopreservation with constant temperatures and temperature fluctuations. a Number of cells capable of adhering to plastic 24 h after thawing. b Typical example of cellular adherence after studied cryopreservation procedures: 1 samples stored at constant temperatures; 2 samples after 50 cycles in the range of temperature fluctuations between –196 °C and –80 °C; 3 native control without cryopreservation. Scale bars = 100 μm