Fig. 4

Stroke alters fiber outgrowth pattern of human skin-derived neural progenitors transplanted adjacent to SVZ. a-c Fluorescence photomicrographs showing fiber outgrowth from grafted cells (GFP+, green) 2 months after transplantation in intact and stroke-injured (MCAO) rats. In the intact brain, grafted cells send massive numbers of fibers through the RMS (a) reaching MOB (b) including granular layer (c) and glomerular layer (d; arrowheads depict GFP+ fibers). e-h Number of fibers crossing the rostral turn of the RMS (e and f right, and g) and reaching the MOB (e and f left, and h), from grafted cells 2 months after transplantation in intact (n = 6) and stroke-subjected (n = 7) rats. Arrows in (e) depict massive number of fibers in the intact brain and arrowheads in (f) depict rare fibers found in the stroke-subjected brains (MCAO). Data represent means ± SEM; *P < 0.05, two-tailed unpaired t test. LV lateral ventricle, RMS rostral migratory stream, MOB main olfactory bulb, GrL granular layer, GL glomerular layer. Scale bars represent 1 mm in (a), 50 μm in (b) also valid for (c and d) and 100 μm in (e and f)