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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: miR199a-3p regulates P53 by targeting CABLES1 in mouse cardiac c-kit+ cells to promote proliferation and inhibit apoptosis through a negative feedback loop

Fig. 4

CABLES1 is a target of miR199a-3p in cardiac c-kit+ cells. a The nucleotide sequence of miR199a-3p, the predicted binding sites of miR199a-3p, and the mutated nucleotides (underlined) in the 3′ UTR of CABLES1. b Dual luciferase activity in transfected cardiac c-kit+ cells. c Regulation of endogenous expression of CABLES1 by treatment with the miR199a-3p lentiviral vectors and anti-miR199a-3p. Analysis of total RNA to determine the relative expression of miR199a-3p targets through qRT-PCR in cardiac c-kit+ cells at 48 h after transfection. Relative expression levels were normalized to GAPDH levels. d Western blot analysis of proteins from cardiac c-kit+ cells transfected with the miR199a-3p lentiviral vectors and anti-miR199a-3p. The protein profiles were normalized to α-actin. CABLES1 Cdk5 and Abl enzyme substrate 1, Con control (*p < 0.05, compared to miR-con)

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