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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Human mesenchymal stromal cells inhibit tumor growth in orthotopic glioblastoma xenografts

Fig. 2

Intratumoral injections of hMSCs in orthotopic xenografts of glioma stem-like cells (GSCs). a Schematic drawing of the intratumoral injection of m-Cherry adipose-derived human mesenchymal stem/stromal cells (Ad-hMSCs) (red) in athymic rats with established brain xenografts of patient-derived GSC1 tumor cells (green). b Low-power fluorescence microphotograph of a coronal section of rat brain through the tumor epicentre showing the injection sites of Ad-hMSCs (arrow). Scale bar = 500 μm. c Microphtograph of GSC1 xenograft immunostained with anti-Iba1 (white staining) showing an area injected with Ad-hMSCs with depletion of tumor cells and microglial reaction. Scale bar = 75 μm. d Bar graph showing the mean density of GSC1 tumor cells in regions injected with Ad-hMSCs (grey bar) and in saline-injected region of control tumors (black bar; ***p < 0.0001). e Detail of interaction between unloaded Ad-hMSCs and GSC1 tumor cells. Scale bar = 25 μm. f Detail of PTX-loaded Ad-hMSCs lying adjacent to a multinucleated GSC1 tumor cell (yellow arrow). Scale bar = 25 μm. gi Microphotographs showing cell proliferation, as assessed by Ki67 labeling, in GSC1 xenografts injected with g unloaded Ad-hMSCs, h with PTX-loaded Ad-hMSCs, and i with saline. The arrows point out proliferating tumor cells. Scale bars = 75 μm

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