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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Cytokine-free directed differentiation of human pluripotent stem cells efficiently produces hemogenic endothelium with lymphoid potential

Fig. 3

T-lymphoid, B-lymphoid, and myeloid potential assessment of CHIR99021-induced HE and OP9 coculture-derived progenitors. a Representative flow cytometry analysis showing the differentiating cells gated on the lymphoid fraction containing the CD3+ T-cell population and the percentage of CD4+CD8+ double-positive T cells after 3 weeks of differentiation. b Representative flow cytometry analysis showing TCRαβ and TCRγδ rearrangements gated on CD3+ cells after 4 weeks of differentiation. c Representative flow cytometry analysis demonstrating the percentage of cells expressing B-lymphoid potential (CD19). d Yield of CD56+, CD3+, and CD19+ cells per 1 × 105 cells plated. Error bars are SEM. e Representative cytometric analysis of cells derived after OP9 coculture induction gated on CD15+ myeloid fraction, and cytospin images of floating cells showing a variety of hematopoietic cell types. f Representative cytometric analysis of cells derived after CHIR99021 induction gated on CD15+ myeloid fraction, and cytospin images of floating cells showing a variety of hematopoietic cell types. TCR T-cell receptor

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