Table 2 Primers for RT-PCR analysis
Number | Oligo name | Sequence (5′–3′) | Primer size |
|---|---|---|---|
1 | Nanog | Forward: CCT CCT CCC ATC CCT CAT | 18 |
Reverse: GGA TGG GCA TCA TGG AAA | 18 | ||
2 | Oct4 | Forward:CCC TCC AGG TGG TGG | 15 |
Reverse:GGC CTT GGAAGC TTA | 15 | ||
3 | Sox2 | Forward: CTC CGG GAC ATG ATC AGC | 18 |
Reverse: AGT GCT GGG ACA TGT GAA | 18 | ||
4 | Vimentin | Forward: AGG ATG TTG ACAATG CGT | 18 |
Reverse: ATC GAT TTG GAC ATG CTG | 18 | ||
5 | HSP47 | Forward: AGC AGC AAG CAG CAC TAC | 18 |
Reverse: AAT TTC TCG TCC CAG TGT | 18 | ||
6 | Desmin | Forward: CAG ACC TAC TCT GCC CTC | 18 |
Reverse: GAT CAT CAC CGT CTT CTT | 18 | ||
7 | B-actin | Forward: TCC ACA TGC TTT ATT CCA | 18 |
Reverse: TGG CAC CCA GCA CAA TGA | 18 |