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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Impaired osteogenesis of T1DM bone marrow-derived stromal cells and periosteum-derived cells and their differential in-vitro responses to growth factor rescue

Fig. 4

High glucose inhibits growth factor rescue of impaired osteogenic differentiation of diabetic BMSCs but not the rescue of osteogenesis of diabetic PDCs. a Optical micrographs (100 × x) and b quantification (n = 4) of alizarin red stain for osteogenesis of diabetic BMSCs and PDCs after 2-week culture in high glucose (25 mM) osteogenic media supplemented with rhBMP-2/7 (45 or 300 ng/ml), rhIGF-1 (15 or 100 ng/ml), or their combination. *p < 0.05 (ANOVA) for pairwise comparisons with that cultured in osteogenic media without growth factor treatment (* directly above the data bars) or between single and dual growth factor treatments (* above the brackets indicating specific data pairs compared); error bars, standard deviation of the mean. O.M. osteogenic media, BMP-2/7 bone morphogenetic protein-2/7 heterodimer, BMSC bone marrow-derived stromal cell, IGF-1 insulin-like growth factor-1, PDC periosteum-derived cell

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Stem Cell Research & Therapy

ISSN: 1757-6512