Fig. 4

Cell cycle-dependent changes in the γH2AX and p53-binding protein 1 (53BP1) foci number. A comparison of the foci number between the G1 phase (Edu negative; black column) and S/G2 phase (EdU positive; grey column) was performed in one fibroblast line (human dermal fibroblast; hDF), three hiPSC lines (CBIA-3, CBIA-5, and CBIA-7) and one hESC line (CCTL-14) at high or low passage number. a The number of foci per cell is shown in non-irradiated control cells (Ctrl) and irradiated cells (1 Gy; 0.5 h after irradiation). b Percentage of increase in γH2AX and 53BP1 foci after 1 Gy treatment. The mean ± SEM is shown. *P ˂ 0.05 by t test