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Table 1 Number of foci per cell

From: DNA double-strand breaks in human induced pluripotent stem cell reprogramming and long-term in vitro culturing

  Non-irradiated control cells Irradiated cells (1Gy)
G1 (EdU-negative cells) S/G2 (EdU-positive cells) G1 (EdU-negative cells) S/G2 (EdU-positive cells)
No. of foci ± SEM No. of cells No. of foci ± SEM No. of cells No. of foci ± SEM No. of cells No. of foci ± SEM No. of cells
hDF
 γH2AX 1.13 ± 0.11 463 29.9 ± 1.32 60 19.89 ± 0.36 485 45.49 ± 0.88 97
 53BP1 1.49 ± 0.07 486 19.64 ± 1.23 80 15.55 ± 0.22 501 19.20 ± 0.91 69
CBIA-3 low
 γH2AX 5.56 ± 0.22 464 22.98 ± 0.38 523 21.87 ± 0.34 612 28.71 ± 0.34 554
 53BP1 2.13 ± 0.12 445 8.76 ± 0.35 407 19.01 ± 0.31 552 23.1 ± 0.42 496
CBIA-3 high
 γH2AX 4.42 ± 0.20 483 17.91 ± 0.41 337 8.01 ± 0.27 594 7.97 ± 0.35 480
 53BP1 1.46 ± 0.07 625 4.73 ± 0.18 510 14.45 ± 0.29 551 13.66 ± 0.31 447
CBIA-5 low
 γH2AX 5.69 ± 0.23 542 25.1 ± 0.47 415 28.6 ± 0.52 437 41.00 ± 0.55 421
 53BP1 4.03 ± 0.18 445 15.3 ± 0.49 425 17.84 ± 0.26 613 23.32 ± 0.30 642
CBIA-5 high
 γH2AX 2.98 ± 0.13 520 10.98 ± 0.26 508 11.57 ± 0.27 445 24.45 ± 0.39 480
 53BP1 1.56 ± 0.07 608 4.71 ± 0.21 461 8.04 ± 0.17 490 10.84 ± 0.20 514
CBIA-7 low
 γH2AX 5.89 ± 0.25 382 23.77 ± 0.45 414 26.48 ± 0.44 511 36.71 ± 0.52 444
 53BP1 2.73 ± 0.13 487 10.42 ± 0.31 421 20.05 ± 0.35 591 21.94 ± 0.38 560
CBIA-7 high
 γH2AX 2.56 ± 0.13 547 11.78 ± 0.25 534 12.41 ± 0.26 580 12.73 ± 0.30 452
 53BP1 1.51 ± 0.07 586 6.5 ± 0.27 415 16.9 ± 0.32 556 17.62 ± 0.34 415
CCTL-14 low
 γH2AX 4.45 ± 0.20 554 12.83 ± 0.31 544 20.99 ± 0.34 634 30.37 ± 0.57 378
 53BP1 2.73 ± 0.14 629 10.28 ± 0.36 508 16.77 ± 0.27 681 19.13 ± 0.39 452
CCTL-14 high
 γH2AX 6.66 ± 0.29 510 19.30 ± 0.49 427 16.26 ± 0.28 642 19.38 ± 0.34 423
 53BP1 4.75 ± 0.21 576 14.34 ± 0.52 402 21.49 ± 0.30 653 24.20 ± 0.42 425
  1. The number of foci per cell and the number of analysed cells are shown for non-irradiated control cells and cells 0.5 h after irradiation with 1 Gy. Five samples were analysed: a fibroblast line (hDF), three hiPSC lines (CBIA-3, CBIA-5, and CBIA-7) and one hESC line (CCTL-14) at high or low passage number. The values are shown for the separated cell cycle phases G1 (EdU-negative) or S/G2 (EdU-positive)
  2. EdU 5-ethynyl-2′-deoxyuridine, hDF human dermal fibroblast