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Table 1 Number of foci per cell

From: DNA double-strand breaks in human induced pluripotent stem cell reprogramming and long-term in vitro culturing

 

Non-irradiated control cells

Irradiated cells (1Gy)

G1 (EdU-negative cells)

S/G2 (EdU-positive cells)

G1 (EdU-negative cells)

S/G2 (EdU-positive cells)

No. of foci ± SEM

No. of cells

No. of foci ± SEM

No. of cells

No. of foci ± SEM

No. of cells

No. of foci ± SEM

No. of cells

hDF

 γH2AX

1.13 ± 0.11

463

29.9 ± 1.32

60

19.89 ± 0.36

485

45.49 ± 0.88

97

 53BP1

1.49 ± 0.07

486

19.64 ± 1.23

80

15.55 ± 0.22

501

19.20 ± 0.91

69

CBIA-3 low

 γH2AX

5.56 ± 0.22

464

22.98 ± 0.38

523

21.87 ± 0.34

612

28.71 ± 0.34

554

 53BP1

2.13 ± 0.12

445

8.76 ± 0.35

407

19.01 ± 0.31

552

23.1 ± 0.42

496

CBIA-3 high

 γH2AX

4.42 ± 0.20

483

17.91 ± 0.41

337

8.01 ± 0.27

594

7.97 ± 0.35

480

 53BP1

1.46 ± 0.07

625

4.73 ± 0.18

510

14.45 ± 0.29

551

13.66 ± 0.31

447

CBIA-5 low

 γH2AX

5.69 ± 0.23

542

25.1 ± 0.47

415

28.6 ± 0.52

437

41.00 ± 0.55

421

 53BP1

4.03 ± 0.18

445

15.3 ± 0.49

425

17.84 ± 0.26

613

23.32 ± 0.30

642

CBIA-5 high

 γH2AX

2.98 ± 0.13

520

10.98 ± 0.26

508

11.57 ± 0.27

445

24.45 ± 0.39

480

 53BP1

1.56 ± 0.07

608

4.71 ± 0.21

461

8.04 ± 0.17

490

10.84 ± 0.20

514

CBIA-7 low

 γH2AX

5.89 ± 0.25

382

23.77 ± 0.45

414

26.48 ± 0.44

511

36.71 ± 0.52

444

 53BP1

2.73 ± 0.13

487

10.42 ± 0.31

421

20.05 ± 0.35

591

21.94 ± 0.38

560

CBIA-7 high

 γH2AX

2.56 ± 0.13

547

11.78 ± 0.25

534

12.41 ± 0.26

580

12.73 ± 0.30

452

 53BP1

1.51 ± 0.07

586

6.5 ± 0.27

415

16.9 ± 0.32

556

17.62 ± 0.34

415

CCTL-14 low

 γH2AX

4.45 ± 0.20

554

12.83 ± 0.31

544

20.99 ± 0.34

634

30.37 ± 0.57

378

 53BP1

2.73 ± 0.14

629

10.28 ± 0.36

508

16.77 ± 0.27

681

19.13 ± 0.39

452

CCTL-14 high

 γH2AX

6.66 ± 0.29

510

19.30 ± 0.49

427

16.26 ± 0.28

642

19.38 ± 0.34

423

 53BP1

4.75 ± 0.21

576

14.34 ± 0.52

402

21.49 ± 0.30

653

24.20 ± 0.42

425

  1. The number of foci per cell and the number of analysed cells are shown for non-irradiated control cells and cells 0.5 h after irradiation with 1 Gy. Five samples were analysed: a fibroblast line (hDF), three hiPSC lines (CBIA-3, CBIA-5, and CBIA-7) and one hESC line (CCTL-14) at high or low passage number. The values are shown for the separated cell cycle phases G1 (EdU-negative) or S/G2 (EdU-positive)
  2. EdU 5-ethynyl-2′-deoxyuridine, hDF human dermal fibroblast