Fig. 5

Isolated CD146⁺ and CD34⁺ cells express MSC markers. qPCR measurements (a) of CD73 (left), CD90 (middle), and CD105 (right) of non-sorted (light gray), CD34⁺ (dark gray), and CD146⁺ (black) cells analyzed at passage 1 or 2 (P1/2) and 3 or 4 (P3/4). Flow cytometry histograms (b) displaying fluorescence intensity (AF488 conjugated to the secondary antibody) vs event counts (corresponding to number of cells) and showing displacement of non-sorted (light gray), CD34⁺ (dark gray), and CD146⁺ (black) cultured cells stained with antibodies CD29, CD44, CD90, and CD105 in comparison with the isotype controls (very light gray, on left). Data were obtained from P3 to P5. c Quantification of the flow signals depicted in bar graphs as mean fluorescence intensity (MFI) of non-sorted (light gray), CD34⁺ (dark gray), and CD146⁺ (black). n ≥ 3; mean ± SEM. *p < 0.05, **p < 0.001, ***p < 0.0001 between means. AU arbitrary units