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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Dysregulated systemic lymphocytes affect the balance of osteogenic/adipogenic differentiation of bone mesenchymal stem cells after local irradiation

Fig. 3

Changes in differentiation of and relative mRNA and protein levels in BMSCs at 12 weeks after irradiation. a ALP, Alizarin Red, and Oil Red O staining of BMSCs. b RUNX2 mRNA expression, (c) PPARγ mRNA expression, and (d) RUNX2/PPARγ ratio. Western blot image (e) and RUNX2 (f) and PPARγ (g) protein expression levels at 12 weeks. Data are presented as the means ± standard deviations; * P < 0.05, ** P < 0.01 and *** P < 0.001 (n = 6/group). ALP alkaline phosphatase, PPARγ peroxisome proliferator-activated receptor γ, RUNX2 runt-related transcription factor 2

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