Fig. 6

Changes in differentiation and relative mRNA and protein levels of BMSCs after co-culture with lymphocytes from irradiated rats. a ALP, Alizarin Red, and Oil Red O staining of co-cultured BMSCs. b RUNX2 mRNA expression, (c) PPARγ mRNA expression, and (d) RUNX2/PPARγ ratio. Western blot image (e) and RUNX2 (f) and PPARγ (g) protein expression levels at 12 weeks. Data are presented as the means ± standard deviations; ^* P < 0.05, ^** P < 0.01, and ^*** P < 0.001 (n = 6/group). ALP alkaline phosphatase, PPARγ peroxisome proliferator-activated receptor γ, RUNX2 runt-related transcription factor 2