Fig. 1From: Effect of cryopreservation on proliferation and differentiation of periodontal ligament stem cell sheetsThe ECM was intact and undisrupted in cPDLSC sheets. Macroscope of cPDLSC sheet (a) and fPDLSC sheet (f) shows that their “sheet-like” structures were intact. H&E staining of cPDLSC sheets (b) and fPDLSC sheets (g) showing a uniform and multilayer tissue structure. Visualization of type I collagen (COLI; c), fibronectin (d), and integrin (e) in cPDLSC sheets by immunostaining. Visualization of type I collagen (h), fibronectin (i), and integrin (j) in fPDLSC sheets by immunostaining. Scale bars = (b,g) 20 μm; (c–e, h–j) 25 μm. Magnification: (b, g) 40×; (c–e, h–j) 100 ×Back to article page