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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Effect of cryopreservation on proliferation and differentiation of periodontal ligament stem cell sheets

Fig. 6

Transplated cryopreserved periodontal ligament stem cell (cPDLSC) sheets could develop into periodontal tissues with a well-structured ECM in nude mice. Hematoxylin and eosin (H&E) staining showed odontoblast-like cells (arrows) and bone-like matrix (solid triangles) developed from transplanted cPDLSC sheets (a) and freshly prepared periodontal ligament stem cell (fPDLSC; b) sheets. Sirius Red staining of the developed tissues showing a network of collagen type I and III proteins in cPDLSC (c) sheets and fPDLSC sheets (d). Visualization of the Sirius Red staining results in polarized light; collagen I and III are indicated in red (arrows) and green (solid triangles), respectively, in cPDLSC sheets (e) and fPDLSC sheets (f). Scale bars = 20 μm. Magnification: 40×

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