Fig. 3

Time-dependent effects of stress on CD99 expression. a Flow cytometry analysis of surface (s) CD99 (recognized by 3B2 mAB) (mean of at least three experiments are shown with SEM) and (b) cytoplasmatic (cy) CD99. ^* P <0.1, ^** P <0.05, ^*** P <0.01, Student’s t test. c Representative picture for sCD99 expression in normoxic (gray profile), starved (blue line), hypoxic (red line), and H/S cells (green line) cultures at day 42. Western blots of CD99 expression in MSC cell line (d) and in primary stromal cells (e). GAPDH – loading control. f RT-PCR analysis of both CD99 isoforms expression, type I and type II