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Table 1 Characteristics of microcarriers tested and their respective seeding conditions per heMSC-microcarrier construct at day 0 of differentiation

From: Critical attributes of human early mesenchymal stromal cell-laden microcarrier constructs for improved chondrogenic differentiation

  Cytodex 1 Cytodex 3 SphereCol Cultispher-S
Microcarrier characteristics
 Diameter (μm) 147–248 141–211 100–400 130–380
 Matrix Dextran Dextran Type I Collagen Gelatin
 Charges/coating Positively charged Denatured collagen
 Porosity Microporous Microporous Microporous Macroporous
 Biodegradability No No Yes Yes
 Surface area (cm2/mg dry weight) 4.40 2.70 15.0
heMSC-microcarrier construct-seeding conditions (spinner culture, data per construct)
 Cell confluency 70% 70%
 Total cell number 10.1 × 103 10.1 × 103
 Microcarrier number 300 300
heMSC-microcarrier construct-seeding conditions (shake flask culture, data per construct)
 Cell confluency 70% 70% 70% 70%
 Total cell number 10.1 × 103 8.88 × 103 8.88 × 103 30.8 × 103
 Microcarrier number 300 300 300 50
  1. heMSC human early mesenchymal stromal cell