Fig. 1

Schematic overview of the muscle-derived stem cell (MDSC) purification process and comparative analysis of nestin expression in PP1 vs PP8 cells. After a 40-min pre-plate to allow fibroblasts to adhere to the plate, nonadherent cells are daily re-plated in new medium and culture dishes over 7 days to produce a nonadherent and proliferative stem cell population—the MDSC. a Schematic diagram of the pre-plating purification process. b Representative photomicrographs of undifferentiated PP8-MDSC by phase-contrast and Hoechst staining for DNA. c Immunostaining analysis for nestin on cytospins of PP0 total muscle cells (left panels) and PP8 nonadherent MDSC (right panels). The histogram summarizes nestin expression in 300–400 cells for each sample (PP0 and PP8) performed after immunofluorescence analysis for nestin on five different MDSC isolation experiments. Scale bars = 20 μm